PureSperm® (NIDACON) Isolation Media
 PureSperm® 100
Improved stock suspension for density gradients
Features of PureSperm® Gradients:
- Increases sperm survival time
- Removes sources of ROS
- Removes bacteria
- Removes viral infectivity (e.g. HIV, hepatitis C)
- Removes sperm with abnormal DNA
- Improves cryo-survival
- Improves yield and recovery compared to swim-up techniques
Safety and Quality:
- Longest shelf life of commercially available gradients
- Pharmaceutical grade packaging to maintain product quality
- Lowest endotoxin levels
- Terminal sterilization by autoclaving
- Cleared by regulatory authorities around the world
- Produced according to cGMP and ISO standards
PureSperm® 100 stock suspension should be diluted with PureSperm® Buffer to provide layers of different densities required for the gradient. For optimum results, the sperm pellet should be washed in PureSperm® Wash. These three products together form an optimized system for preparation of sperm for ART. Return to Top  PureSperm® 40/80
All the advantages of a PureSperm® gradient PLUS ready-diluted to save preparation time!
PureSperm® 40 and PureSperm® 80 are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential.
PureSperm®40 and PureSperm® 80 maintain pH between 7.4 - 7.8 at room temperature and at 37°, providing suitable conditions for good sperm motility, survival time, and fertilization potential.
The preparation contains glucose as a usable energy substrate for normal cell metabolism and sperm function.
The gradient enables motile sperm to be separated from extraneous cells and seminal plasma. In addition, damage to sperm DNA and structural molecules cause by reactive oxygen species (ROS) is minimized by the effective removal of immature sperm and lymphocytes. Bacteria, epithelial cells, and cell debris are also separated from the motile sperm, which can be difficult to achieve in polysucrose based gradient preparations.
Return to Top  PureSperm® Buffer
The ideal diluent for PureSperm®
PureSperm® Buffer is a balanced salt solution designed specifically for diluting PureSperm® 100 to make up layers of different densities for a gradient. The optimized formula of PureSperm® Buffer is designed to maximize sperm survival during density gradient centrifugation.
Why use PureSperm® Buffer?
Previously there was no specific buffer available to dilute PureSperm® when making different density layers required for a gradient.
Due to popular demand, we are now offering a salt solution which has been optimized for use with either PureSperm® or PureSperm®100.
Now, clinicians can select the density layers they want to use, in any combination. Return to Top  PureSperm® Wash
An optimized salt solution specifically for washing the sperm pellet after purification on a PureSperm® gradient
PureSperm® Wash is a balanced salt solution containing EDTA and human serum albumin. PureSperm® Wash is designed specifically for washing the sperm pellet obtained from a PureSperm® gradients.
Previously there was no specific washing solution available to wash the sperm pellet after purification on a PureSperm® gradient.
We now offer a balanced salt solution which has been specifically formulated for this purpose. Our customers can choose an integrated approach to sperm preparation, by using products which have been optimized to match each other and fit the specific needs of the sperm.
Return to Top  ReadySwim™
Specifically for swim-up, optimized, ready to use
What is "swim-up?"
In vivo, motile sperm quickly migrate from liquefying semen into the cervix, thereby removing themselves from the presences of both seminal plasma and reactive oxygen species arising from cell debris. In the andrology laboratory, this process can be imitated by allowing the sperm to swim into a layer of medium, the so-called "swim-up" method, thereby separating them from the remainder of the ejaculate.
Excellent sperm recovery, motility, and survival
The formulation of ReadySwim™ is ionically balanced to avoid premature hyperactivation. Sudden pH and osmolarity changes
during the movement of sperm from the semen sample to the fertilization medium are minimized. Avoiding premature hyperactivation conserves energy resources, and improves fertilization potential.
Return to Top  SpermAssist™
To re-suspend sperm for intrauterine insemination
SpermAssist™ is a HEPES-buffered salt solution which has been formulated specifically to resuspend sperm for intra-uterine insemination. The ionic balance in SpermAssist™ has been designed to meet the requirements of the sperm while avoiding premature hyperactivation.
Sperm survival and fertilizing capacity, and therefore also pregnancy rates, can be improved by avoiding media which cause premature hyperactivation.
SpermAssist™ does not contain any coloring agents or preservatives, which can be toxic to sperm.
Return to Top  SpermCatch™
SpermCatch™ is a natural alternative to polyvinyl plastic for slowing sperm for ICSI.
Until now, the only products available for slowing sperm motility contained polyvinylpyrrolidone. Clinicians are concerned that injecting this artificial product into the oocyte along with the sperm may cause damage to acrosomal, mitochondrial and plasma membranes, as well as to the chromosomal DNA.
Now clinicians have an alternative method for modulating sperm motility.
SpermCatch™ contains only substances normally found in the mammalian reproductive tract. Exposure of sperm to SpermCatch™, not only slows sperm, but also mimics the naturally occurring sequence of events during in vivo fertilization.
Return to Top  NidOil™
In vitro overlay for gametes and zygotes
Some procedures for IVF and ICSI use an oil overlay to protect small volumes of culture medium from evaporation in the incubator and to help dampen the effects of sudden changes in temperature, gas saturation, and pH. Likewise, gamete manipulation under the microscope requires the gametes to be held in small drops of medium under oil, to maintain a constant environment within the culture medium.
Now NidaCon® offers you NidOil™, a paraffin oil product designed with gametes and embryos in mind. The oil is neither sticky nor too viscous, to facilitate pipetting, but is sufficiently viscous to prevent movement of the drops of media around the culture dish.
Return to Top Sperm CryoProtec™
 For cryopreserving human sperm
Freezing Sperm
Freezing solutions for sperm traditionally contain egg yolk and glycerol. Clinicians are now questioning the wisdom of exposing human sperm for ART to exogenous sources of protein, such as egg yolk. Furthermore, high concentration of glycerol can be toxic to sperm.
Innovative Formulation
NidaCon® listens to clinicians: we have developed a new cryoprotectant for human sperm, free from egg yolk and containing a reduced concentration of glycerol.
What is Sperm CryoProtec™
It is an optimized solution solution for protecting human sperm from the damaging effects of freezing and thawing. The solution contains osmotically active ingredients to reduce intracellular water, and cryoprotectants to reduce injury caused by ice crystal formation. Human serum albumin has been added as an exipient to: (i) serve as a cryoprotectant, (ii) counteract the detrimental effects of reactive oxygen species, and (iii) prevent sperm aggregation and adherence to laboratory equipment.
Return to Top  PureSperm® SpeediKit
Many clinicians in small clinics or private practices are looking for a safe and effective method of preparing sperm fro intra-uterine insemination.
They are unsure of their ability to layer a density gradient correctly to achieve optimal results. However, they also know the "swim-up" does not remove sperm with abnormal morphology or damaged DNA, and does not separate out bacteria and viruses from the sperm preparation. So what should the use?
Now there is an alternative method of preparing human sperm for IUI - the PureSperm® SpeediKit. The kit contains sufficient sperm preparation materials for ten patients, already dispensed in Type 1 glass centrifuge tubes. In addition, we provide 10 semen collective tubes, one for each patient, and empty tubes to balance the centrifuge rotor. (NOTE: The 5 sample kit is temporarily unavailable.)
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