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Oosight™ Imaging System from CRi

Non-invasively reveal the spindle and zona pellucida in living oocytes

The CRi Oosight™ Imaging System is a next-generation, improved version of CRi's own, original, industry-leading SpindleView™ system. Use the system for assisted reproductive technology applications for imaging the meiotic and mitotic spindle, the three-layered zona pellucida, and other birefringent structures. Assess the quality of living oocytes. Non-invasively locate and position the spindle for ICSI. Monitor changes in spindle parameters from oocyte to oocyte. Remove the spindle in nuclear transfer techniques without using Hoechst dye DNA staining, thus minimizing damage to vital cytoplasmic organelles. Track the changing molecular organization of the zona pellucida through embryo development.

Applications

  • Identify and position the spindle during ICSI
  • Assess oocyte quality
  • Locate the spindle for nuclear transfer
  • Assess freezing effects on the spindle
  • Study zona pellucida, cytoplasm and sperm morphology
  • Please visit the CRi web site for detailed descriptions of applications

Features & Benefits

  • Real-time imaging (both through the eyepieces and on the computer screen)
  • No setup procedure required (no sample preparation, no system calibration or lamp adjustment necessary)
  • Birefringence data automatically displayed at every pixel
  • Single-click image acquisition
  • Easy-to-use, Windows™-based software interface
  • Time-lapse digital movie capture option
  • Compatible with ZILOS-tk laser system and others

Components

  • Oosight software for direct viewing, image capture and retardance calculations
  • Liquid crystal universal compensator
  • Circular polarizer
  • 546-nm interference filter
  • Scientific-grade CCD camera with fast USB 2.0 interface
  • Computer system and 17" LCD Monitor (optional)
Microscope required but not included.

Additional Infomation

Ooosight Brochure (PDF)

This Downloadable PDF Image Gallery (10 MB) shows stunning detail of the meiotic spindle and trilaminar zona pellucida both in MI anaphase/telophase oocytes as well as in MII oocytes. Images courtesy of RMANJ and RBA, Atlanta.

Annotated References

Please see the CRi web site for additional information and video clip.

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