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Accu-beads® for Quality Control of CASA and Manual Sperm Counts
What Are Accu-Beads?Accu-Beads are the original sperm count quality control product developed by Hamilton Thorne Biosciences. The Accu-Beads quality control system consists of two known concentrations of latex beads (18 and 35 M/ml) suspended in an aqueous solution. Similar in size to human sperm, the Accu-Beads have been developed to confirm accurate counting procedures used for routine semen analysis. Who Will Benefit from Using Accu-Beads?Whether you perform your semen counts manually or using an automated analyzer, you can use Accu-Beads for quality control. What do Accu-Beads Evaluate?For manual methods, the Accu-Beads are used to evaluate the actual counting procedures practiced. When used in an automated semen analyzer, the Accu-Beads test and evaluate the optical and image acquisition systems of the analyzer. Because motility is irrelevant for checking these functions, the static nature of the Accu-Beads will not affect the quality control testing. How are Accu-Beads Used?
Additional InformationAccu-Beads are packaged in 5 ml aliquots and are stable at room temperature for up to 1 year. Complete instructions for use with the Hamilton Thorne Research automated analyzers and for manual counting methods are included with each package. Download Accu-beads Instructions View Accu-beads Certificates of Analysis (on Hamilton Thorne Biosciences web site)
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Using a pipette, simply draw the required amount (undiluted) and load your standard semen analysis chamber, such as a disposable slide or a Petroff-Hausser, Makler, or Hemacytometer chamber. Position the chamber on the stage of the microscope or analyzer and perform an analysis according to your normal procedure.